@article{6543d680b7f14b26b4db8260ec022879,
title = "Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation",
abstract = "Mitotic chromosome assembly remains a big mystery in biology. Condensin complexes are pivotal for chromosome architecture yet how they shape mitotic chromatin remains unknown. Using acute inactivation approaches and live-cell imaging in Drosophila embryos, we dissect the role of condensin I in the maintenance of mitotic chromosome structure with unprecedented temporal resolution. Removal of condensin I from pre-established chromosomes results in rapid disassembly of centromeric regions while most chromatin mass undergoes hyper-compaction. This is accompanied by drastic changes in the degree of sister chromatid intertwines. While wild-type metaphase chromosomes display residual levels of catenations, upon timely removal of condensin I, chromosomes present high levels of de novo Topoisomerase II (TopoII)-dependent re- entanglements, and complete failure in chromosome segregation. TopoII is thus capable of re- intertwining previously separated DNA molecules and condensin I continuously required to counteract this erroneous activity. We propose that maintenance of chromosome resolution is a highly dynamic bidirectional process.",
author = "E. Piskadlo and A. Tavares and R.A. Oliveira",
note = "Funding Information: We thank S Heidmann, H Bellen and B Suter for fly strains, antibodies and plasmids, I Telley, A Atha- nasiadis for helpful advice, the Advance Imaging Unit and fly facility for technical assistance, all the members of the RAO laboratory for discussions and comments, and Kim Nasmyth for comments on the manuscript. EP holds a PhD fellowship from the Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (SRFH/ BD/52172/2013). This work was supported by the following grants awarded to RAO: FCT Investiga- tor grant (IF/00851/2012/CP0185/CT0004 WP1), Marie Curie Career Integration Grant (MCCIG321883/CCC), EMBO Installation Grant (IG2778) and European Research Council Starting Grant (ERC-2014-STG-638917). Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia SRFH/BD/52172/2013 Ewa Piskadlo European Commission MCCIG321883/CCC Raquel A Oliveira European Molecular Biology Organization IG2778 Raquel A Oliveira Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia a IF/00852012/CP0185/ Raquel A Oliveira Tecnologia CT0004 WP1 European Commission ERC-2014-STG-638917- ChromoCellDev Raquel A Oliveira Publisher Copyright: {\textcopyright} Piskadlo et al.",
year = "2017",
month = may,
day = "6",
doi = "10.7554/eLife.26120",
language = "English",
volume = "6",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd",
}