Modulated stress to balance Nannochloropsis oculata growth and eicosapentaenoic acid production

Sérgio Sousa, Ana C. Freitas, Ana M. Gomes*, Ana P. Carvalho

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

Resumo

Two environmental parameters, temperature and light intensity, were independently used as stress modulators to enhance eicosapentaenoic acid (EPA) production by the microalga Nannochloropsis oculata, without hindering biomass production. A sinusoidal approach was used, as environmental conditions were alternated between optimum and stress status in multi-day cycles. Low temperatures (5 and 10 °C) and light intensities (30 and 50 μmol photons/m2/s) were tested. Results revealed that the modulated stress approach used was able to avoid decreases in biomass production. Temperature stress (10 °C) presented the highest impact, increasing EPA content to 12.8 mgEPA/L, 158% more than the amount obtained in optimum (non-modulated) growth conditions at that point in time, while the lower light intensity stress was able to increase to 126% more. It is important to point out that in both cases increases in EPA amounts resulted from increased content in each individual cell and not just from increased biomass contents. Key points: • Temperature stress (10 °C) presented the highest impact increasing EPA content 158% • Lower light intensity stress was able to increase EPA to 126% more • EPA increased in individual cell contents simultaneous with biomass increase.
Idioma originalEnglish
Páginas (de-até)4017-4027
Número de páginas11
RevistaApplied Microbiology and Biotechnology
Volume106
Número de emissão11
DOIs
Estado da publicaçãoPublished - 23 mai 2022

Impressão digital

Mergulhe nos tópicos de investigação de “Modulated stress to balance Nannochloropsis oculata growth and eicosapentaenoic acid production“. Em conjunto formam uma impressão digital única.

Citação