TY - CHAP
T1 - Online combination of bioassays with chemical and structural characterization for detection of bioactive compounds
AU - Freitas, Ana C.
AU - Montalvão, Sofia Isabel G.H.M.
AU - Duarte, Armando C.
AU - Rocha-Santos, Teresa
PY - 2014
Y1 - 2014
N2 - Simultaneous screening and chemical characterization of bioactive compounds in complex matrices such as marine natural products have been attempted in recent years using a number of methodological approaches based on high-throughput screening (HTS) or on high-resolution screening (HRS), each with its own advantages and drawbacks. These screening systems integrate separation and chemical detection methods with biochemical assays. This chapter focuses primarily on studies of marine products and examines both HTS and HRS screening systems, involving total or partial online screening. The principles and studies of HTS methods based on precolumn and postcolumn methodologies are presented and discussed. Hyphenated techniques, coupling online separation and biochemical detection assays for screening and identifying bioactive compounds, in turn, are presented and discussed in the second part of the chapter.
AB - Simultaneous screening and chemical characterization of bioactive compounds in complex matrices such as marine natural products have been attempted in recent years using a number of methodological approaches based on high-throughput screening (HTS) or on high-resolution screening (HRS), each with its own advantages and drawbacks. These screening systems integrate separation and chemical detection methods with biochemical assays. This chapter focuses primarily on studies of marine products and examines both HTS and HRS screening systems, involving total or partial online screening. The principles and studies of HTS methods based on precolumn and postcolumn methodologies are presented and discussed. Hyphenated techniques, coupling online separation and biochemical detection assays for screening and identifying bioactive compounds, in turn, are presented and discussed in the second part of the chapter.
KW - High-resolution screening
KW - High-throughput screening
KW - Marine bioactive compounds
KW - Online biochemical detection assays
UR - https://www.scopus.com/pages/publications/84906562376
U2 - 10.1016/b978-0-444-63359-0.00010-0
DO - 10.1016/b978-0-444-63359-0.00010-0
M3 - Chapter
AN - SCOPUS:84906562376
T3 - Comprehensive Analytical Chemistry
SP - 253
EP - 278
BT - Comprehensive analytical chemistry
PB - Elsevier Science B.V.
ER -