TY - JOUR
T1 - Optimisation, by response surface methodology, of degree of hydrolysis and antioxidant and ACE-inhibitory activities of whey protein hydrolysates obtained with cardoon extract
AU - Tavares, T. G.
AU - Contreras, M. M.
AU - Amorim, M.
AU - Martín-Álvarez, P. J.
AU - Pintado, M. E.
AU - Recio, I.
AU - Malcata, F. X.
PY - 2011/12
Y1 - 2011/12
N2 - The hydrolysis of bovine whey protein concentrate (WPC), α-lactalbumin (α-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated parameters. The model was statistically appropriate to describe ACE-inhibitory activity of hydrolysates from WPC and α-La, but not from CMP. Maximum DH was 18% and 9%, for WPC and α-La, respectively. 50% ACE-inhibition was produced by 105.4 (total fraction) and 25.6μgmL-1 (<3kDa fraction) for WPC, and 47.6 (total fraction) and 22.5μgmL-1 (<3kDa fraction) for α-La. Major peptides of fractions exhibiting ACE-inhibition were sequenced. The antioxidant activities of WPC and α-La were 0.96±0.08 and 1.12±0.13μmoltrolox equivalent per mghydrolysed protein, respectively.
AB - The hydrolysis of bovine whey protein concentrate (WPC), α-lactalbumin (α-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated parameters. The model was statistically appropriate to describe ACE-inhibitory activity of hydrolysates from WPC and α-La, but not from CMP. Maximum DH was 18% and 9%, for WPC and α-La, respectively. 50% ACE-inhibition was produced by 105.4 (total fraction) and 25.6μgmL-1 (<3kDa fraction) for WPC, and 47.6 (total fraction) and 22.5μgmL-1 (<3kDa fraction) for α-La. Major peptides of fractions exhibiting ACE-inhibition were sequenced. The antioxidant activities of WPC and α-La were 0.96±0.08 and 1.12±0.13μmoltrolox equivalent per mghydrolysed protein, respectively.
UR - http://www.scopus.com/inward/record.url?scp=80052907959&partnerID=8YFLogxK
U2 - 10.1016/j.idairyj.2011.05.013
DO - 10.1016/j.idairyj.2011.05.013
M3 - Article
AN - SCOPUS:80052907959
SN - 0958-6946
VL - 21
SP - 926
EP - 933
JO - International Dairy Journal
JF - International Dairy Journal
IS - 12
ER -