P82 Peptide fraction identification by SE-HPLC and LC-MS/MS analysis of the body mucus from Portugal coastal fish Halobatrachus didactylus

M. Cunha*, E. R. Coscueta, M. E. Brassesco, F. Almada, D. Gonçalves, M. Manuela Pintado

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

15 Transferências (Pure)

Resumo

The mucus covers the fish's body, working as a protective barrier. Besides physical protection, mucus provides molecules that protect the fish from pathogens damaging 1,2. These include antimicrobial peptides secreted in the mucus, which play an essential role in defense against microbial pathogens since these belong to the innate immune system2,3. In this study, two adult Halobatrachus didactylus individuals were captured from the wild in Sesimbra. Then, mucus collection was performed by scraping the dorsal-lateral body of the fish with a sponge. Our objective was the identification of new peptides with bioactive potential in mucus samples by chromatography analysis. Size exclusion highperformance liquid chromatography (SE-HPLC) analysis performed on mucus samples from the two individuals revealed a similar profile with an intense highlight peak which resulted in a distribution of about 775 Dalton. With interest in that peak, the two mucus samples were pooled for fractionation by SEC. The resulting fraction was analyzed by liquid chromatography-tandem mass spectrometry (LCMS/MS) to identify the most probable peptide sequences. Identification from databases did not provide reliable results, indicating a lack of information on the matrix analyzed. We resorted to de novo sequencing with good results using PEAKS Studio software. Five identified peptides were selected according to their bioactivities predicted in silico. Furthermore, the five identified peptides were synthesized, and the molecular size was validated by SE-HPLC analysis. Overall, this chromatographic approach enabled the identification of promising peptides, which bioactivities will be evaluated in vitro in future work.
Idioma originalEnglish
Título da publicação do anfitriãoLivro de resumos do 12º Encontro Nacional de Cromatografia e XIV WARPA
EditoresSílvia M. Rocha
Local da publicaçãoLisboa
EditoraSociedade Portuguesa de Química
Páginas208-208
Número de páginas1
ISBN (eletrónico)9789898124371
Estado da publicaçãoPublicado - 6 dez. 2022
Evento12º Encontro Nacional de Cromatografia - Universidade de Aveiro, Aveiro
Duração: 6 dez. 20228 dez. 2022
https://12enc.events.chemistry.pt/

Conferência

Conferência12º Encontro Nacional de Cromatografia
Título abreviado12ENC
País/TerritórioPortugal
CidadeAveiro
Período6/12/228/12/22
Endereço da Internet

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