Protective effect of a DNA vaccine cocktail encoding ROP13 and GRA14 with Alum nano-adjuvant against Toxoplasma gondii infection in mice

Abdol Sattar Pagheh, Ahmad Daryani, Paria Alizadeh, Hadi Hassannia, Sonia M. Rodrigues Oliveira, Tohid Kazemi, Fatemeh Rezaei, Maria de Lourdes Pereira, Ehsan Ahmadpour*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

9 Citações (Scopus)

Resumo

Toxoplasma gondii is an obligate intracellular protozoan parasite that can cause serious public health problems. The development of a safe and effective vaccine against T. gondii is urgently needed to prevent and control the spread of toxoplasmosis. The aim of this study was to evaluate the immune responses induced by a pcGRA14 + pcROP13 vaccine cocktail in BALB/c mice. All groups were immunized intramuscularly three times at two-week intervals. The production of anti-Toxoplasma gondii lysate antigen (TLA) antibodies, lymphocyte proliferation, serum levels of IFN-γ and IL-4 cytokines and the survival time were monitored after vaccination and challenged with the virulent RH strain of T. gondii. The results showed that immunization with the pcGRA14 + pcROP13 DNA vaccine significantly increased the production of specific IgG antibodies and cytokines against toxoplasmosis. Interestingly, high levels of IgG2a and IFN-γ were found in animals vaccinated with DNA vaccine cocktail. Furthermore, immunized mice challenged with the RH strain of T. gondii showed prolonged survival time when compared to control groups (P <0.05). The present study demonstrates the potential of a DNA cocktail vaccine expressing pcGRA14 and pcROP13 in developing specific immune responses and providing effective protection against T. gondii infection.
Idioma originalEnglish
Número do artigo105920
Número de páginas10
RevistaInternational Journal of Biochemistry and Cell Biology
Volume132
Data online antecipadamar. 2021
DOIs
Estado da publicaçãoPublicado - mar. 2021
Publicado externamenteSim

Impressão digital

Mergulhe nos tópicos de investigação de “Protective effect of a DNA vaccine cocktail encoding ROP13 and GRA14 with Alum nano-adjuvant against Toxoplasma gondii infection in mice“. Em conjunto formam uma impressão digital única.

Citação