Protein and DNA nanoparticulate multiantigenic vaccines against H. pylori: In vivo evaluation

Lara Figueiredo*, Cecilia C. R. Calado, António J. Almeida, Lídia M. D. Gonçalves

*Autor correspondente para este trabalho

    Resultado de pesquisarevisão de pares

    6 Citações (Scopus)

    Resumo

    Immunisation against H. pylori is an attractive option for antibiotic resistance and reinfection situations. Strain genetic heterogeneity, and low immunogenicity of protein antigens and DNA alone are nonetheless obstacles to this approach. We developed multigenic H. pylori DNA-nanoparticle and protein-nanoparticle vaccines based on pathogenic relevance. Six antigens were chosen for the vaccine construction: CagA, VacA, HpaA, UreB, HomB and GroEL. Different combinations of CS/DS and CS/Alg /TPP nanoparticles with DNA and chimeric proteins were produced as vaccine systems. Immune responses were evaluated after i.m. and oral immunisation of BALB/c mice. Oral vaccination successfully stimulated mucosal immunity while i.m. immunisation efficiently elicited a more equilibrated cellular/humoral immune response.
    Idioma originalEnglish
    Título da publicação do anfitrião2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
    DOIs
    Estado da publicaçãoPublished - 2012
    Evento2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012 - Coimbra
    Duração: 23 fev 201225 fev 2012

    Série de publicação

    Nome2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012

    Conferência

    Conferência2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
    País/TerritórioPortugal
    CidadeCoimbra
    Período23/02/1225/02/12

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