RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: adulterant sheep breeds in Serra da Estrela cheese production

Joana T. Cunha, Tânia I. B. Ribeiro, João B. Rocha, João Nunes, José A. Teixeira, Lucília Domingues*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

27 Citações (Scopus)

Resumo

Serra da Estrela Protected Designation of Origin (PDO) cheese is the most famous Portuguese cheese and has a high commercial value. However, the adulteration of production with cheaper/lower-quality milks from non-autochthones ovine breeds compromises the quality of the final product and undervalues the original PDO cheese. A Randomly Amplified Polymorphic DNA (RAPD) method was developed for efficient detection of adulterant breeds in milk mixtures used for fraudulent production of this cheese. Furthermore, Sequence Characterized Amplified Region (SCAR) markers were designed envisioning the detection of milk adulteration in processed dairy foods. The RAPD-SCAR technique is here described, for the first time, to be potentially useful for detection of milk origin in dairy products. In this sense, our findings will play an important role on the valorization of Serra da Estrela cheese, as well as on other high-quality dairy products prone to adulteration, contributing to the further development of the dairy industry.

Idioma originalEnglish
Páginas (de-até)631-636
Número de páginas6
RevistaFood Chemistry
Volume211
DOIs
Estado da publicaçãoPublicado - 15 nov. 2016
Publicado externamenteSim

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