@article{ef376214c59047c4b027fc14c5a8d269,
title = "RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: adulterant sheep breeds in Serra da Estrela cheese production",
abstract = "Serra da Estrela Protected Designation of Origin (PDO) cheese is the most famous Portuguese cheese and has a high commercial value. However, the adulteration of production with cheaper/lower-quality milks from non-autochthones ovine breeds compromises the quality of the final product and undervalues the original PDO cheese. A Randomly Amplified Polymorphic DNA (RAPD) method was developed for efficient detection of adulterant breeds in milk mixtures used for fraudulent production of this cheese. Furthermore, Sequence Characterized Amplified Region (SCAR) markers were designed envisioning the detection of milk adulteration in processed dairy foods. The RAPD-SCAR technique is here described, for the first time, to be potentially useful for detection of milk origin in dairy products. In this sense, our findings will play an important role on the valorization of Serra da Estrela cheese, as well as on other high-quality dairy products prone to adulteration, contributing to the further development of the dairy industry.",
keywords = "Milk adulteration, Ovine breeds, Quality control, RAPD, SCAR, Serra da Estrela cheese",
author = "Cunha, {Joana T.} and Ribeiro, {T{\^a}nia I. B.} and Rocha, {Jo{\~a}o B.} and Jo{\~a}o Nunes and Teixeira, {Jos{\'e} A.} and Luc{\'i}lia Domingues",
note = "Funding Information: The authors acknowledge Paulo Rog{\'e}rio Sim{\~o}es Figueiredo, Lda for supplying milk samples. This work was financially supported by the project “ Valor Queijo ” (CENTRO-07-0202-FEDER-030372) funded by FCT/MCTES (PIDDAC) and co-funded by “ Fundo Europeu de Desenvolvimento Regional ” (FEDER) through “COMPETE – Programa Operacional Factores de Competitividade” (POFC). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the Strategic Project of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and the project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd.",
year = "2016",
month = nov,
day = "15",
doi = "10.1016/j.foodchem.2016.05.109",
language = "English",
volume = "211",
pages = "631--636",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier Ltd.",
}