Reverse hydrolysis by cardosin A: specificity considerations

A. Cristina Sarmento, Cláudia Oliveira, Euclides Pires, Francisco Amado, Marlene Barros*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

12 Citações (Scopus)

Resumo

Cardosin A, a plant aspartic proteinase, capable of synthesising peptides, was investigated through synthesis of five methyl esters amino acid substrates as amino donors and nine benzyloxycarbonyl amino acid and peptide carboxyl donors. It was found that cardosin A is able to catalyse the synthesis of several peptide bonds, being the preference order for the carboxyl components the following: CBz.Phe>CBz.Trp. Unpredictably, Tyr could not be accepted in P1. Results were compared and discussed according to the known specificity of pepsin, the most studied aspartic proteinase.

Idioma originalEnglish
Páginas (de-até)33-37
Número de páginas5
RevistaJournal of Molecular Catalysis B: Enzymatic
Volume28
Número de emissão1
DOIs
Estado da publicaçãoPublicado - 2 abr. 2004

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