Stability performance of Cynara cardunculus L. acid protease in aqueous-organic biphasic systems

M. T. Barros, M. G. V. Carvalho, F. A. P. Garcia, E. M. V. Pires*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

11 Citações (Scopus)

Resumo

Stability performance of the acid protease of Cynara cardunculus L. in biphasic systems containing ethyl acetate, n-hexane or isooctane was investigated and compared with that of pepsin. Activity retention was higher in the system containing n-hexane. In this system 100% retention was observed up to 144 hours. Pre-saturation of phases was found to increase enzyme stability in the cases of n-hexane and isooctane and to be an absolute requirement in the case of ethyl acetate. The results obtained suggest also that, when dealing with pre-saturated phases, log P cannot be used straightforwardly to predict enzyme stability in biphasic systems.
Idioma originalEnglish
Páginas (de-até)179-184
Número de páginas6
RevistaBiotechnology Letters
Volume14
Número de emissão3
DOIs
Estado da publicaçãoPublicado - mar 1992
Publicado externamenteSim

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