Supercritical CO2 assisted process for the production of high-purity and sterile nano-hydroxyapatite/chitosan hybrid scaffolds

G. Ruphuy, M. Souto-Lopes, D. Paiva, P. Costa, Alírio Egídio Rodrigues, F. J. Monteiro, C. L. Salgado, M. H. Fernandes, J. C. Lopes, M. M. Dias, M. F. Barreiro*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

11 Citações (Scopus)


Hybrid scaffolds composed of hydroxyapatite (HAp), in particular in its nanometric form (n-HAp), and chitosan (CS) are promising materials for non-load-bearing bone graft applications. The main constraints of their production concern the successful implementation of the final purification/neutralization and sterilization steps. Often, the used purification strategies can compromise scaffold structural features, and conventional sterilization techniques can result in material's thermal degradation and/or contamination with toxic residues. In this context, this work presents a process to produce n-HAp/CS scaffolds mimicking bone composition and structure, where an innovative single step based on supercritical CO2 extraction was used for both purification and sterilization. A removal of 80% of the residual acetic acid was obtained (T = 75°C, p = 8.0 MPa, 2 extraction cycles of 2 h) giving rise to scaffolds exhibiting adequate interconnected porous structure, fast swelling and storage modulus compatible with non-load-bearing applications. Moreover, the obtained scaffolds showed cytocompatibility and osteoconductivity without further need of disinfection/sterilization procedures. Among the main advantages, the proposed process comprises only three steps (n-HAp/CS dispersion preparation; freeze-drying; and supercritical CO2 extraction), and the supercritical CO2 extraction show clear advantages over currently used procedures based on neutralization steps.

Idioma originalEnglish
Páginas (de-até)965-975
Número de páginas11
RevistaJournal of Biomedical Materials Research - Part B Applied Biomaterials
Número de emissão3
Estado da publicaçãoPublicado - abr 2018
Publicado externamenteSim

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