The Impact of Host Diet on Wolbachia Titer in Drosophila

Laura R. Serbus*, Pamela M. White, Jessica Pintado Silva, Amanda Rabe, Luis Teixeira, Roger Albertson, William Sullivan

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

58 Citações (Scopus)

Resumo

While a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.

Idioma originalEnglish
Número do artigoe1004777
Páginas (de-até)1-25
Número de páginas25
RevistaPLoS Pathogens
Volume11
Número de emissão3
DOIs
Estado da publicaçãoPublicado - 1 mar 2015
Publicado externamenteSim

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