TY - CHAP
T1 - Untargeted metabolomics relative quantification by swath mass spectrometry applied to cerebrospinal fluid
AU - Mendes, Vera M.
AU - Coelho, Margarida
AU - Manadas, Bruno
N1 - Funding Information:
This work was supported by Fundac¸ão para a Ciência e Tecnologia (FCT) (PTDC/SAU-NMC/112183/2009, PTDC/NEU-NMC/0205/2012, PTDC/NEU-SCC/7051/2014, UID/-NEU/04539/2013, UID/BIM/04773/2013, PEst-C/SAU/ LA0001/2013-2014, POCI-01-0145-FEDER-016428 (ref.: SAICTPAC/0010/2015), POCI-01-0145-FEDER-029311 (ref.: PTDC/BTM-TEC/29311/2017), POCI-01-0145-FEDER-30943 (ref.: PTDC/MEC-PSQ/30943/2017)), PTDC/MED-NEU/27946/2017 and co-financed by “COMPETE Programa Operacional Factores de Competitividade”, QREN; the European Union (FEDER—Fundo Europeu de Desenvolvimento Regional); and by The National Mass Spectrometry Network (RNEM) (POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013)REDE/1506/REM/2005). Mar-garida Coelho acknowledges FCT for the doctoral fellowship PD/BD/135178/2017 integrated in the PhD Program in NMR applied to chemistry, materials, and biosciences (PD/00065/ 2013).
Funding Information:
This work was supported by Funda??o para a Ci?ncia e Tecnologia (FCT) (PTDC/SAU-NMC/112183/2009, PTDC/NEUNMC/0205/2012, PTDC/NEU-SCC/7051/2014, UID/NEU/04539/2013, UID/BIM/04773/2013, PEst-C/SAU/ LA0001/2013-2014, POCI-01-0145-FEDER-016428 (ref.: SAICTPAC/0010/2015), POCI-01-0145-FEDER-029311 (ref.: PTDC/BTM-TEC/29311/2017), POCI-01-0145FEDER-30943 (ref.: PTDC/MEC-PSQ/30943/2017)), PTDC/MED-NEU/27946/2017 and co-financed by ?COMPETE Programa Operacional Factores de Competitividade?, QREN; the European Union (FEDER?Fundo Europeu de Desenvolvimento Regional); and by The National Mass Spectrometry Network (RNEM) (POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013)REDE/1506/REM/2005). Margarida Coelho acknowledges FCT for the doctoral fellowship PD/BD/135178/2017 integrated in the PhD Program in NMR applied to chemistry, materials, and biosciences (PD/00065/ 2013).
Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - Cerebrospinal fluid (CSF) has been considered the key source for the search of biomarkers, in particular for neurological diseases, such as Alzheimer’s and Parkinson’s disease, since it reflects the state of the central nervous system (CNS). Finding biomarkers in the earliest stages of neurodegenerative diseases has become imperative, since, at the moment, there are no drugs that can reverse these pathological processes. Untargeted metabolomics analysis by liquid chromatography combined with SWATH-MS relative quantification is an emerging approach to search for potential biomarkers. In this chapter, we describe a method for untargeted metabolomics analysis of CSF samples that can also be used in parallel to a proteomics approach. The analysis is focused on the SWATH acquisition mode, where beyond precursor’s relative quantification, the information of the MS/MS relative quantification is also used to help in the search of new potential biomarkers.
AB - Cerebrospinal fluid (CSF) has been considered the key source for the search of biomarkers, in particular for neurological diseases, such as Alzheimer’s and Parkinson’s disease, since it reflects the state of the central nervous system (CNS). Finding biomarkers in the earliest stages of neurodegenerative diseases has become imperative, since, at the moment, there are no drugs that can reverse these pathological processes. Untargeted metabolomics analysis by liquid chromatography combined with SWATH-MS relative quantification is an emerging approach to search for potential biomarkers. In this chapter, we describe a method for untargeted metabolomics analysis of CSF samples that can also be used in parallel to a proteomics approach. The analysis is focused on the SWATH acquisition mode, where beyond precursor’s relative quantification, the information of the MS/MS relative quantification is also used to help in the search of new potential biomarkers.
KW - Cerebrospinal fluid
KW - Potential biomarkers
KW - SWATH-MS relative quantification
KW - Untargeted metabolomics
UR - https://www.scopus.com/pages/publications/85071534256
U2 - 10.1007/978-1-4939-9706-0_20
DO - 10.1007/978-1-4939-9706-0_20
M3 - Chapter
C2 - 31432422
AN - SCOPUS:85071534256
SN - 9781493997053
T3 - Methods in Molecular Biology
SP - 321
EP - 336
BT - Methods in molecular biology
PB - Humana Press Inc.
ER -