The growth and viability of Bifidobacterium lactis and Lactobacillus acidophilus per se and for both strains as a coculture were studied in reconstituted skim milk so as to assess their dependence upon temperature (5, 10 and 15C), NaCl concentration (0 0.51 and 1.03 mol L-1), and storage time (0-8 weeks). Cultures of B. lactis exhibited no significant loss of viability either with increasing NaCl concentration or increasing storage temperature within the ranges studied. On the other hand, viability of L. acidophilus decreased with increasing temperature and increasing NaCl concentration under similar conditions. When cocultured with L. acidophilus, B. lactis was significantly less tolerant to higher NaCl levels and higher temperatures than when in pure culture, although final numbers were still above the threshold required for commercial application. Coculturing with B. lactis had no detrimental effect on viability of L. acidophilus. A mechanistic model, which considers the behavior of the pure and mixed microbial populations is described by specific death rates which vary with temperature (following Arrhenius relationships) and NaCl levels (following simple inhibition kinetics) in the milk medium. Activation energies for the death rates of B. lactis and L. acidophilus were 14 kcal mol-1 and 9-15 kcal mol-1, respectively.
|Número de páginas
|Journal of Food Processing and Preservation
|Número de emissão
|Estado da publicação
|Publicado - ago. 1998